Review



protoscript m mulv taq rt pcr system  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    New England Biolabs protoscript m mulv taq rt pcr system
    Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time <t>RT-PCR</t> analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).
    Protoscript M Mulv Taq Rt Pcr System, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+m+mulv+taq+rt+pcr+system/pmc11055028-53-26-31
    Average 86 stars, based on 1 article reviews
    protoscript m mulv taq rt pcr system - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma"

    Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics16040566

    Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time RT-PCR analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).
    Figure Legend Snippet: Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time RT-PCR analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).

    Techniques Used: Expressing, Flow Cytometry, Confocal Microscopy, Western Blot, Quantitative RT-PCR, Binding Assay, Comparison, Labeling, Fluorescence, Software

    Related Articles

    cDNA Synthesis:

    Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer′s instructions. .. After DNase treatment with RQ1 RNase-free DNase (Promega, Mannheim, Germany), 1 μg of total RNA was used for first-strand cDNA synthesis with oligo-dT primers using the ProtoScript M-MuLV Taq RT-PCR system (New England Biolabs, MA, USA). .. Q-PCR amplification was performed in a Quantstudio 7 real-time PCR system (Applied Biosystems, CA, USA) using 96-well plates.

    Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma.
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer′s instructions. .. After DNase treatment with RQ1 RNase-free DNase (Promega, Mannheim, Germany), 1 μg of total RNA was used for firststrand cDNA synthesis with oligo-dT primers using the ProtoScript M-MuLV Taq RT-PCR system (New England Biolabs, MA, USA). .. Q-PCR amplification was performed in a Quantstudio 7 real-time PCR system (Applied Biosystems, CA, USA) using 96-well plates.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer′s instructions. .. After DNase treatment with RQ1 RNase-free DNase (Promega, Mannheim, Germany), 1 μg of total RNA was used for first-strand cDNA synthesis with oligo-dT primers using the ProtoScript M-MuLV Taq RT-PCR system (New England Biolabs, MA, USA). .. Q-PCR amplification was performed in a Quantstudio 7 real-time PCR system (Applied Biosystems, CA, USA) using 96-well plates.

    Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma.
    Article Snippet: Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer′s instructions. .. After DNase treatment with RQ1 RNase-free DNase (Promega, Mannheim, Germany), 1 μg of total RNA was used for firststrand cDNA synthesis with oligo-dT primers using the ProtoScript M-MuLV Taq RT-PCR system (New England Biolabs, MA, USA). .. Q-PCR amplification was performed in a Quantstudio 7 real-time PCR system (Applied Biosystems, CA, USA) using 96-well plates.



    Similar Products

    protoscript m mulv taq rt pcr system  (New England Biolabs)
    86
    New England Biolabs protoscript m mulv taq rt pcr system
    Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time <t>RT-PCR</t> analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).
    Protoscript M Mulv Taq Rt Pcr System, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+m+mulv+taq+rt+pcr+system/pmc11055028-53-26-31
    Average 86 stars, based on 1 article reviews
    protoscript m mulv taq rt pcr system - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    protoscript m mulv taq rt pcr kit  (New England Biolabs)
    98
    New England Biolabs protoscript m mulv taq rt pcr kit
    Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time <t>RT-PCR</t> analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).
    Protoscript M Mulv Taq Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+m+mulv+taq+rt+pcr+system/Taq+PCR+Kit/us12496358-694-13-18
    Average 98 stars, based on 1 article reviews
    protoscript m mulv taq rt pcr kit - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    protoscript tm m mulv taq rt pcr kit  (New England Biolabs)
    98
    New England Biolabs protoscript tm m mulv taq rt pcr kit
    Reagents and tools table
    Protoscript Tm M Mulv Taq Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+m+mulv+taq+rt+pcr+system/Taq+PCR+Kit/pmc11169469-570-33-42
    Average 98 stars, based on 1 article reviews
    protoscript tm m mulv taq rt pcr kit - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    protoscript moloney murine leukemia virus m mulv taq rt pcr kit  (New England Biolabs)
    98
    New England Biolabs protoscript moloney murine leukemia virus m mulv taq rt pcr kit
    Reagents and tools table
    Protoscript Moloney Murine Leukemia Virus M Mulv Taq Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protoscript+m+mulv+taq+rt+pcr+system/Taq+PCR+Kit/pmc10344723-70-11-20
    Average 98 stars, based on 1 article reviews
    protoscript moloney murine leukemia virus m mulv taq rt pcr kit - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time RT-PCR analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).

    Journal: Pharmaceutics

    Article Title: Nucleolin-Targeting AS1411 Aptamer-Conjugated Nanospheres for Targeted Treatment of Glioblastoma

    doi: 10.3390/pharmaceutics16040566

    Figure Lengend Snippet: Nucleolin mRNA and protein expression analysis and flow cytometry and confocal microscopy images of non-target NHA cells and target U87 and U251 glioblastoma cells treated with nanosphere and Apt-Nanosphere. ( A ) Western blot analysis of NCL mRNA expression level of NHA, U87, and U251 cells; β-actin was used as an internal control. ( B ) Real-time RT-PCR analysis of NCL mRNA expression level of NHA, U87, and U251 cells; GAPDH was used as an internal control. Selective binding of AS1411 Apt-Nanosphere to target the U87 ( C ) and 251 glioblastoma cells ( D ) and non-target NHA cells ( E ). ( F ) A comparison of the binding affinity of the FAM-labeled nanospheres and 1411 aptamer-conjugated FAM-labeled nanospheres in the target U87 and 251 glioblastoma cells, as well as the non-target NHA cells. The scale bar for all images is 20 µm. ( G ) The mean fluorescence intensity values were calculated using the ZEN lite software. The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 6). Relative quantification was performed using the comparative Ct method (2 −ΔΔCt ). The values are expressed as mean ± SEM: ** p < 0.01 and * p < 0.05 ( n = 4).

    Article Snippet: After DNase treatment with RQ1 RNase-free DNase (Promega, Mannheim, Germany), 1 µg of total RNA was used for first-strand cDNA synthesis with oligo-dT primers using the ProtoScript M-MuLV Taq RT-PCR system (New England Biolabs, MA, USA).

    Techniques: Expressing, Flow Cytometry, Confocal Microscopy, Western Blot, Quantitative RT-PCR, Binding Assay, Comparison, Labeling, Fluorescence, Software

    Reagents and tools table

    Journal: EMBO Reports

    Article Title: Conserved role of hnRNPL in alternative splicing of epigenetic modifiers enables B cell activation

    doi: 10.1038/s44319-024-00152-3

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: RNA was isolated using TRIzol (Life Technologies) or TRI-reagent (Molecular Research Center, Inc), following the manufacturer’s instruction, and quantified by NanoDrop (Thermo Fisher). cDNA was synthesized from 1 μg of RNA using the ProtoScript TM M-MuLV Taq RT-PCR kit and random primers (New England BioLabs).

    Techniques: Isolation, Recombinant, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Sequencing, shRNA, Control, Adjuvant, cDNA Synthesis, Software, Western Blot, Real-time Polymerase Chain Reaction, Imaging